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MCA NHS Ester（AMCA NHS酯）介紹：
MCA NHS Ester（琥珀酰-7-氨基-4-甲基香豆素-3-乙酸）是最流行的藍(lán)色熒光標(biāo)記分子之一。MCA NHS Ester 廣泛用于標(biāo)記抗體、蛋白質(zhì)和藥物小分子。 通常用作免疫熒光顯微鏡、陣列和原位雜交中雙重和三重標(biāo)記的對(duì)比探針。 AMCA 染料的理想特性包括相對(duì)較大的斯托克位移和抗光漂白性。


AMCA NHS酯 在 pH 7-9 下與伯胺（例如，賴氨酸殘基的側(cè)鏈或氨基硅烷涂層表面）特異性且有效地反應(yīng)，形成穩(wěn)定的共價(jià)酰胺鍵。 NHS 酯（或琥珀酰亞胺酯）是將染料與蛋白質(zhì)或抗體 (Lys) 的伯胺、胺修飾的寡核苷酸和其他含胺分子結(jié)合的最常用工具。

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AMCA NHS Ester (succinimydyl-7-amino-4-methylcoumarin-3-acetic acid) is one of the most popular blue fluorescent tagging molecules. It is widely used to label antibodies, proteins and small drug molecules. Often used as contrasting probes for double- and triple-labeling in immunofluorescence microscopy, arrays and in situ hybridization. The desirable properties of AMCA dyes include a relatively large Stoke’s shift and resistance to photobleaching.

AMCA NHS Ester reacts specifically and efficiently with a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a stable, covalent amide bond. The NHS ester (or succinimidyl ester) is the most popular tool for conjugating dyes to the primary amines of protein or antibody (Lys), amine-modified oligonucleotides, and other amine-containing molecules.

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AMCA NHS Ester光譜圖

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