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LB培養(yǎng)基的配方：

試劑	重量
Tryptone	10 grams
NaCl	10 grams
Yeast extract	5 grams
Distilled water	Up to 1 L

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LB培養(yǎng)基應(yīng)用說明Application Notes
LB Medium is a nutritionally rich medium originally developed for growth and maintenance of E. coli strains. The ultimate in ease and convenience, LB Medium in a capsule form eliminates the waste, inaccuracies, and mess associated with weighing out bulk powder. The growth characteristics are the same as LB agar powder. LB Medium supports a luxurious growth of E. coli cells as tryptone and yeast extract supply essential growth factors that E. coli cells would otherwise have to synthesize. The medium also contains essential electrolytes for transport and osmotic balance, due to the NaCl component.

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LB培養(yǎng)基使用注意事項(xiàng)：
1.實(shí)驗(yàn)前需戴好防護(hù)眼鏡，穿戴防護(hù)服和口罩，佩戴手套，避免與皮膚接觸。
2.實(shí)驗(yàn)過程中如遇到有毒或者刺激性物質(zhì)及有害物質(zhì)產(chǎn)生，必要時(shí)實(shí)驗(yàn)操作需要手套箱內(nèi)完成以免對(duì)實(shí)驗(yàn)人員造成傷害。
3.取樣品的移液槍頭需及時(shí)更換，必要時(shí)為避免交叉污染盡可能選擇濾芯吸頭。
4.稱量藥品時(shí)選用稱量紙，并無風(fēng)處取藥和稱量以免揚(yáng)撒，試劑的容器使用前務(wù)必確保干凈，并消毒。
5.取藥品時(shí)盡量采用多個(gè)藥勺分別使用，使用后清洗干凈。6.
實(shí)驗(yàn)后產(chǎn)生的廢棄物需分類存儲(chǔ)，并交于專業(yè)生物廢氣物處理公司處理，以免造成環(huán)境污染。Experimental considerations:
1. Wear protective glasses, protective clothing and masks, gloves, and avoid contact with the skin during the experiment.
2. The waste generated after the experiment needs to be stored separately, and handed over to a professional biological waste gas treatment company to avoid environmental pollution.