1.Pyriproxyfen cyclodextrin inclusion compounds    Journal of Inclusion Phenomena and Macrocyclic Chemistry    2015
Abstract：Pyriproxyfen is a pyridine based pesticide which is effective against mosquito larvae. Here we report the inclusion complexes (ICs) between guest pyriproxyfen and host β and γ cyclodextrins, but not with α cyclodextrin, based on WAXD, DSC, FTIR, and TGA characterization results . We show that one pyriproxyfen molecule is covered with between one and two β or γ cyclodextrin molecules when it forms columnar ICs upon precipitation from aqueous solution.
2.Sterilising effects of pyriproxyfen on Anopheles arabiensis and its potential use in malaria control/Caroline Harris, Dickson W Lwetoijera, Stefan Dongus, Nancy S Matowo, Lena M Lorenz, Gregor J Devine & Silas Majambere/Parasites & Vectors volume 6, Article number: 144 (2013)
Abstract：
(1)Background
Insecticide resistance poses a major threat to current vector control campaigns. Insecticides with novel modes of action are therefore in high demand. Pyriproxyfen (PPF), a conventional mosquito pupacide, has a unique mode of action that also sterilises adult mosquitoes (unable to produce viable offspring) upon direct contact. However, the timing of PPF exposure in relation to when mosquitoes take a blood meal has an important impact on that sterilisation. This study investigated the relationship between different blood feeding and PPF exposure timings to determine the potential of PPF sterilisation in controlling Anopheles arabiensis.
(2)Methods
Four treatment regimens were investigated: blood fed three days before PPF exposure (A), blood fed one day before PPF exposure (B), blood fed one day after PPF exposure (C) and blood fed three days after PPF exposure (D) for their impact on egg laying (fecundity) and the production of viable offspring (fertility), while the impact of PPF exposure on mosquito survival was investigated in the absence of a blood meal. All regimens and the survival study exposed mosquitoes to PPF via the bottle assay at 3 mg AI/m2 for 30 minutes.
(3)Results
Female mosquitoes that blood-fed one day prior to PPF exposure (regimen B), produced no viable offspring during that gonotrophic cycle (100% reduction in fertility). All other treatments had no significant effect. The observed reductions in fecundity and fertility were caused by the retention of eggs (97% of eggs retained, ie produced in the ovaries but not laid, in regimen B, p = 0.0004). Some of these retained eggs were deformed in shape. PPF exposure on mosquito survival in the absence of a blood meal was found to have no effect.
(4)Conclusions
The results presented here suggest that sterilising adult malaria vectors using PPF could form part of a malaria control strategy, taking advantage of the lack of reported resistance to PPF in mosquitoes and its unique mode of action. We propose that targeting resting mosquitoes, which are highly susceptible to PPF at low doses, is the optimal direction for developing this control tool.