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Hoechst 34580 可以被 405 nm 的紫色激光激發(fā),并在約 440 nm 的發(fā)射最大值附近發(fā)射藍(lán)色/青色熒光。 Hoechst 34580具有細(xì)胞滲透性
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HCR207321-50mg 50mg 97.8% ¥ 2850.00 ¥ 2850.00 2-3days
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HCR207321-10mg 10mg 97.8% ¥ 1580.00 ¥ 1580.00 880 2-3days
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HCR207321-5mg 5mg 97.8% ¥ 990.00 ¥ 990.00 580 2-3days
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中文別名 Hoechst 34580(911004-45-0),熒光染料
英文別名 Hoechst 34580(911004-45-0),Hoechst-34580,Hoechst34580
CAS號(hào) 911004-45-0
Inchi InChI=1S/C27H29N7.3ClH/c1-32(2)20-7-4-18(5-8-20)26-28-22-10-6-19(16-24(22)30-26)27-29-23-11-9-21(17-25(23)31-27)34-14-12-33(3)13-15-34;;;/h4-11,16-17H,12-15H2,1-3H3,(H,28,30)(H,29,31);3*1H
InchiKey IPMZLKUUQSFUTA-UHFFFAOYSA-N
分子式 Formula C27H29N7. 3HCl
分子量 Molecular Weight 451.5733646
溶解度Solubility Soluble in H2O : 2.67 mg/mL
性狀 Solid power
儲(chǔ)藏條件 Storage conditions storage at -4℃ (1-2weeks), longer storage period at -20℃ (1-2years)

Hoechst 34580使用計(jì)算:

普通儲(chǔ)備溶液的制備
Table 1. Volume of Water needed to reconstitute specific mass of Hoechst 34580 *CAS 911004-45-0* to given concentration.
Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

  0.1 mg 0.5 mg 1 mg 5 mg 10 mg
1 mM 178.269 µL 891.345 µL 1.783 mL 8.913 mL 17.827 mL
5 mM 35.654 µL 178.269 µL 356.538 µL 1.783 mL 3.565 mL
10 mM 17.827 µL 89.135 µL 178.269 µL 891.345 µL 1.783 mL

The Hoechst stains are a family of fluorescent stains for labeling DNA in fluorescence microscopy. Because these fluorescent stains label DNA, they are also commonly used to visualize nuclei and mitochondria. Hoechst 34580 can be excited by violet laser at 405 nm, and emit blue/cyan fluorescence light around an emission maximum at ~440 nm. It is cell-permeable and provide an excellent tool for flow cytometers equiped with a 405 nm violet laser source.
Hoechst 34580(911004-45-0)實(shí)驗(yàn)注意事項(xiàng):
1.實(shí)驗(yàn)前需戴好防護(hù)眼鏡,穿戴防護(hù)服和口罩,佩戴手套,避免與皮膚接觸。
2.實(shí)驗(yàn)過(guò)程中如遇到有毒或者刺激性物質(zhì)及有害物質(zhì)產(chǎn)生,必要時(shí)實(shí)驗(yàn)操作需要手套箱內(nèi)完成以免對(duì)實(shí)驗(yàn)人員造成傷害
3.實(shí)驗(yàn)后產(chǎn)生的廢棄物需分類存儲(chǔ),并交于專業(yè)生物廢氣物處理公司處理,以免造成環(huán)境污染
Experimental considerations:
1. Wear protective glasses, protective clothing and masks, gloves, and avoid contact with the skin during the experiment.
2. The waste generated after the experiment needs to be stored separately, and handed over to a professional biological waste gas treatment company to avoid environmental pollution.
產(chǎn)品說(shuō)明 Hoechst 34580 (911004-45-0)可以被 405 nm 的紫色激光激發(fā),并在約 440 nm 的發(fā)射最大值附近發(fā)射藍(lán)色/青色熒光。 Hoechst 34580具有細(xì)胞滲透性
IntroductionHoechst 34580(911004-45-0):only use for non-medical purposes in industrial or scientific research, and not for clinical diagnosis and treatment of humans or animals. This product is not medicinal or edible.
Application1
Application2
Application3

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MDA5 Induces a Stronger Interferon Response than RIG-I to GCRV Infection through a Mechanism Involving the Phosphorylation and Dimerization of IRF3 and IRF7 in CIK Cells
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Dual-functionalized graphene oxide for enhanced siRNA delivery to breast cancer cells
Authors: Imani, Rana and Shao, Wei and Taherkhani, Samira and Emami, Shahriar Hojjati and Prakash, Satya and Faghihi, Shahab
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A C-terminal acidic domain regulates degradation of the transcriptional coactivator Bob1
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Fatty acid synthase and its mRNA concentrations are decreased at different times following Hoechst 33342-induced apoptosis in BC3H-1 myocytes
Authors: Zhang X, Kiechle FL.
Journal: Ann Clin Lab Sci (2006): 185


Usefulness of a triple fluorochrome combination Merocyanine 540/Yo-Pro 1/Hoechst 33342 in assessing membrane stability of viable frozen-thawed spermatozoa from Estonian Holstein AI bulls
Authors: Hallap T, Nagy S, Jaakma U, Johannisson A, Rodriguez-Martinez H.
Journal: Theriogenology (2006): 1122


Acid-base and electronic structure-dependent properties of Hoechst 33342
Authors: Aleman C, Namba AM, Casanovas J.
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An in vitro study of Hoechst 33342 redistribution and its effects on cell viability
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Evidence for a qualitative hierarchy within the Hoechst-33342 'side population' (SP) of murine bone marrow cells
Authors: Robinson SN, Seina SM, Gohr JC, Kuszynski CA, Sharp JG.
Journal: Bone Marrow Transplant (2005): 807

Flow cytometric characterization of viable meiotic and postmeiotic cells by Hoechst 33342 in mouse spermatogenesis
Authors: Bastos H, Lassalle B, Chicheportiche A, Riou L, Testart J, Allem and I, Fouchet P.
Journal: Cytometry A (2005): 40

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